Prolonged Viral RNA Detection in Blood and Lymphoid Tissues from Coxsackievirus B4 E2 Orally‐Inoculated Swiss Mice
Identifieur interne : 005408 ( Main/Exploration ); précédent : 005407; suivant : 005409Prolonged Viral RNA Detection in Blood and Lymphoid Tissues from Coxsackievirus B4 E2 Orally‐Inoculated Swiss Mice
Auteurs : Hela Jaïdane [France, Tunisie] ; Jawhar Gharbi [Tunisie] ; Pierre-Emmanuel Lobert [France] ; Bernadette Lucas [France] ; Raïda Hiar [Tunisie] ; Manel Ben M'Hadheb [Tunisie] ; Fabienne Brilot [Belgique] ; Vincent Geenen [Belgique] ; Mahjoub Aouni [Tunisie] ; Didier Hober [France]Source :
- Microbiology and Immunology [ 0385-5600 ] ; 2006-12.
English descriptors
- Teeft :
- Chru lille, Coxsackievirus, Current study, Cvb3 nancy, Cvb4, Diabetes mellitus, Didier hober, Different times, Enterovirus, Experimental model, Experimental models, Gapdh mrna, Intestine, Intraperitoneal route, Lille, Mice inoculated, Natural route, Negative results, Oral inoculation, Oral route, Other tissues, Outbred mice, Pancreas, Paul boulanger, Peripheral blood, Persistent infection, Positive results, Primer sense, Reaction inhibitors, Results show, Small intestine, Spleen, Thymic, Thymus, Various organs, Various times, Viral, Viral infection, Virol.
Abstract
The spreading of viral RNA within Swiss Albino mice orally inoculated with coxsackievirus B4 E2 strain (CVB4 E2) was studied by using RT‐PCR and semi‐nested‐RT‐PCR methods. Viral RNA was detected in various organs: pancreas, heart, small intestine, spleen, thymus, and blood at various postinfectious (p.i.) times ranging from 8 hr to 150 days. Our results show that (i) outbred mice can be infected with CVB4 E2 following an oral inoculation, which results in systemic spreading of viral RNA, (ii) CVB4 E2 infection can be associated with a prolonged detection of viral RNA in spleen, thymus and blood, up to 70 days p.i. and further in other organ tissues.
Url:
DOI: 10.1111/j.1348-0421.2006.tb03874.x
Affiliations:
- Belgique, France, Tunisie
- Hauts-de-France, Nord-Pas-de-Calais, Province de Liège, Région wallonne
- Lille, Liège
- Université Lille 2, Université de Liège
Links toward previous steps (curation, corpus...)
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- to stream Istex, to step Curation: 001155
- to stream Istex, to step Checkpoint: 001261
- to stream Main, to step Merge: 005554
- to stream Main, to step Curation: 005408
Le document en format XML
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Monastir</wicri:regionArea><wicri:noRegion>Monastir</wicri:noRegion></affiliation></author><author><name sortKey="Hober, Didier" sort="Hober, Didier" uniqKey="Hober D" first="Didier" last="Hober">Didier Hober</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Service de Virologie/UPRES EA3610, Faculté de Médecine, Université Lille 2, Bâtiment Paul Boulanger, CHRU Lille, 59037, Lille</wicri:regionArea><placeName><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Nord-Pas-de-Calais</region><settlement type="city">Lille</settlement></placeName><orgName type="university">Université Lille 2</orgName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Microbiology and Immunology</title><title level="j" type="alt">MICROBIOLOGY AND IMMUNOLOGY</title><idno type="ISSN">0385-5600</idno><idno type="eISSN">1348-0421</idno><imprint><biblScope unit="vol">50</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="971">971</biblScope><biblScope unit="page" to="974">974</biblScope><biblScope unit="page-count">4</biblScope><date type="published" when="2006-12">2006-12</date></imprint><idno type="ISSN">0385-5600</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0385-5600</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Chru lille</term><term>Coxsackievirus</term><term>Current study</term><term>Cvb3 nancy</term><term>Cvb4</term><term>Diabetes mellitus</term><term>Didier hober</term><term>Different times</term><term>Enterovirus</term><term>Experimental model</term><term>Experimental models</term><term>Gapdh mrna</term><term>Intestine</term><term>Intraperitoneal route</term><term>Lille</term><term>Mice inoculated</term><term>Natural route</term><term>Negative results</term><term>Oral inoculation</term><term>Oral route</term><term>Other tissues</term><term>Outbred mice</term><term>Pancreas</term><term>Paul boulanger</term><term>Peripheral blood</term><term>Persistent infection</term><term>Positive results</term><term>Primer sense</term><term>Reaction inhibitors</term><term>Results show</term><term>Small intestine</term><term>Spleen</term><term>Thymic</term><term>Thymus</term><term>Various organs</term><term>Various times</term><term>Viral</term><term>Viral infection</term><term>Virol</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">The spreading of viral RNA within Swiss Albino mice orally inoculated with coxsackievirus B4 E2 strain (CVB4 E2) was studied by using RT‐PCR and semi‐nested‐RT‐PCR methods. Viral RNA was detected in various organs: pancreas, heart, small intestine, spleen, thymus, and blood at various postinfectious (p.i.) times ranging from 8 hr to 150 days. Our results show that (i) outbred mice can be infected with CVB4 E2 following an oral inoculation, which results in systemic spreading of viral RNA, (ii) CVB4 E2 infection can be associated with a prolonged detection of viral RNA in spleen, thymus and blood, up to 70 days p.i. and further in other organ tissues.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>France</li><li>Tunisie</li></country><region><li>Hauts-de-France</li><li>Nord-Pas-de-Calais</li><li>Province de Liège</li><li>Région wallonne</li></region><settlement><li>Lille</li><li>Liège</li></settlement><orgName><li>Université Lille 2</li><li>Université de Liège</li></orgName></list><tree><country name="France"><region name="Hauts-de-France"><name sortKey="Jaidane, Hela" sort="Jaidane, Hela" uniqKey="Jaidane H" first="Hela" last="Jaïdane">Hela Jaïdane</name></region><name sortKey="Hober, Didier" sort="Hober, Didier" uniqKey="Hober D" first="Didier" last="Hober">Didier Hober</name><name sortKey="Hober, Didier" sort="Hober, Didier" uniqKey="Hober D" first="Didier" last="Hober">Didier Hober</name><name sortKey="Lobert, Pierre Mmanuel" sort="Lobert, Pierre Mmanuel" uniqKey="Lobert P" first="Pierre-Emmanuel" last="Lobert">Pierre-Emmanuel Lobert</name><name sortKey="Lucas, Bernadette" sort="Lucas, Bernadette" uniqKey="Lucas B" first="Bernadette" last="Lucas">Bernadette Lucas</name></country><country name="Tunisie"><noRegion><name sortKey="Jaidane, Hela" sort="Jaidane, Hela" uniqKey="Jaidane H" first="Hela" last="Jaïdane">Hela Jaïdane</name></noRegion><name sortKey="Aouni, Mahjoub" sort="Aouni, Mahjoub" uniqKey="Aouni M" first="Mahjoub" last="Aouni">Mahjoub Aouni</name><name sortKey="Gharbi, Jawhar" sort="Gharbi, Jawhar" uniqKey="Gharbi J" first="Jawhar" last="Gharbi">Jawhar Gharbi</name><name sortKey="Hiar, Raida" sort="Hiar, Raida" uniqKey="Hiar R" first="Raïda" last="Hiar">Raïda Hiar</name><name sortKey="M Hadheb, Manel Ben" sort="M Hadheb, Manel Ben" uniqKey="M Hadheb M" first="Manel Ben" last="M'Hadheb">Manel Ben M'Hadheb</name></country><country name="Belgique"><region name="Région wallonne"><name sortKey="Brilot, Fabienne" sort="Brilot, Fabienne" uniqKey="Brilot F" first="Fabienne" last="Brilot">Fabienne Brilot</name></region><name sortKey="Geenen, Vincent" sort="Geenen, Vincent" uniqKey="Geenen V" first="Vincent" last="Geenen">Vincent Geenen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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